“…The RNAi-mediated down-regulation of UGT71T5 caused a strong reduction in the levels of puerarin in P. lobata hairy roots ( Adolfo et al., 2022 ), confirming that PlUGT43 (UGT71T5) functions as a C -GT at least partially for puerarin biosynthesis in P. lobata . Interestingly, when the 2-HIS (2-hydroxyisoflavanone synthase; see its place in the pathway in Figure 2 ), which is the entry enzyme catalyzing the formation of isoflavonoid backbone ( Steele et al., 1999 ; Jung et al., 2000 ), was down-regulated in P. lobata hairy roots, the potential C -glycosides of isoliquiritigenin and/or liquiritigenin significantly accumulated, when compared to that in the control roots ( Adolfo et al., 2022 ). This data clearly supports that introduction of the C -glucosyl group can take place at the chalcone stage and then the 2-HIS would be able to accommodate the C -glycosides as substrates ( Figure 2 ).…”
Section: Identification Of Ugts and Omts Acting On Isoflavonoids In ...mentioning confidence: 70%
“…et al., 2023 ), indicating that if the C -glycosylation for puerarin biosynthesis occurs at the isoflavanone stage, it is probably catalyzed by a phylogenetically distinct UGT. A very recent labeling study provided evidence that both isoliquiritigenin and daidzein could be incorporated into puerarin in vivo ( Adolfo et al., 2022 ), suggesting that the 8- C -glycosylation can happen at either the chalcone or isoflavone stage, or simultaneously at both levels.…”
Section: Identification Of Ugts and Omts Acting On Isoflavonoids In ...mentioning confidence: 99%
“…Through the in vitro assays, PlUGT43 was found to have no or negligible activity with the chalcone intermediate isoliquiritigenin ( Wang et al., 2017 ). However, its isoform (officially named UGT71T5), which shares 99.72% sequence identity with PlUGT43, was recently reported to be capable of catalyzing the C -glycosylation activity against both daidzein and isoliquiritigenin ( Adolfo et al., 2022 ). Incubation of the recombinant PlUGT43 or UGT71T5 with 2-hydroxyisoflavanone did not generate a product matching the 2-hydroxyisoflavanone C -glycoside ( Wang et al., 2017 ; Adolfo et al., 2022 ), indicating that they had no C -glycosylation activity with 2-hydroxyisoflavanone.…”
Section: Identification Of Ugts and Omts Acting On Isoflavonoids In ...mentioning confidence: 99%
“…However, its isoform (officially named UGT71T5), which shares 99.72% sequence identity with PlUGT43, was recently reported to be capable of catalyzing the C -glycosylation activity against both daidzein and isoliquiritigenin ( Adolfo et al., 2022 ). Incubation of the recombinant PlUGT43 or UGT71T5 with 2-hydroxyisoflavanone did not generate a product matching the 2-hydroxyisoflavanone C -glycoside ( Wang et al., 2017 ; Adolfo et al., 2022 ), indicating that they had no C -glycosylation activity with 2-hydroxyisoflavanone. The RNAi-mediated down-regulation of UGT71T5 caused a strong reduction in the levels of puerarin in P. lobata hairy roots ( Adolfo et al., 2022 ), confirming that PlUGT43 (UGT71T5) functions as a C -GT at least partially for puerarin biosynthesis in P. lobata .…”
Section: Identification Of Ugts and Omts Acting On Isoflavonoids In ...mentioning confidence: 99%
“…Incubation of the recombinant PlUGT43 or UGT71T5 with 2-hydroxyisoflavanone did not generate a product matching the 2-hydroxyisoflavanone C -glycoside ( Wang et al., 2017 ; Adolfo et al., 2022 ), indicating that they had no C -glycosylation activity with 2-hydroxyisoflavanone. The RNAi-mediated down-regulation of UGT71T5 caused a strong reduction in the levels of puerarin in P. lobata hairy roots ( Adolfo et al., 2022 ), confirming that PlUGT43 (UGT71T5) functions as a C -GT at least partially for puerarin biosynthesis in P. lobata . Interestingly, when the 2-HIS (2-hydroxyisoflavanone synthase; see its place in the pathway in Figure 2 ), which is the entry enzyme catalyzing the formation of isoflavonoid backbone ( Steele et al., 1999 ; Jung et al., 2000 ), was down-regulated in P. lobata hairy roots, the potential C -glycosides of isoliquiritigenin and/or liquiritigenin significantly accumulated, when compared to that in the control roots ( Adolfo et al., 2022 ).…”
Section: Identification Of Ugts and Omts Acting On Isoflavonoids In ...mentioning confidence: 99%
See 4 more Smart Citations
Li,
Zhang
2023
Front. Plant Sci.
The pathway for forming isoflavonoid skeletal structure is primarily restricted to the Leguminosae family. Subsequent decorations on the compound backbone by tailoring enzymes would change their biological and medicinal properties. Pueraria lobata is a leguminous plant, and as a traditional Chinese medicine its roots have been ascribed a number of pharmacological activities. Glycosylation and methylation are the main modifying processes in isoflavonoid metabolism in P. lobata roots, resulting in the accumulation of unique glycosylated and methylated end isoflavonoid compounds. For instance, daidzein 8-C-glucoside (i.e., puerarin) and puerarin derivatives are produced only by the Pueraria genus. Puerarin has been established as a clinical drug for curing cardiovascular diseases. To better understand the characteristic isoflavonoid metabolism in P. lobata, this review attempts to summarize the research progress made with understanding the main glycosylation and methylation of isoflavonoids in P. lobata and their biosynthetic enzymes.
